CyTOF - Mass Cytometry
CyTOF is located on floor 10 of Core Facility
CyTOF lab tel: 010-1033697
Facility Manager: Florence Sjögren, tel: 010-1037718 (mini call 0740 556602)
Operator: Mikael Pihl, tel: 010-1032292
Platform Director: Jan-Ingvar Jönsson
The Flow Cytometry Unit is home to Sweden's first Mass Cytometer, a CyTOF 2 from DVS Sciences / Fluidigm, which is a part of the national resource for mass cytometry. Mass Cytometry substitutes Lanthanide isotopes for fluorochromes and separates them by mass using time-of-flight measurement. Problems with background signal due to autofluorescence and limitations due to spectral overlap are thus eliminated. There are approximately 40 different isotopes available that can be conjugated to antibodies, as well as live/dead staining options. It is however not possible to acquire morphological data like on a traditional cytometer.
Linköping is a part of Science for Life Laboratory, Affinity Proteomics.
Read about CyTOF in Forskning och Utveckling. (p22-23)
Contact Jan-Ingvar Jönsson for further information.
Last updated: Mon Dec 15 15:29:09 CET 2014